Novel Imaging Modalities Shedding Light on PlantBiology: Start Small and Grow Big

by Natalie M. Clark, Lisa Van den Broeck, Marjorie Guichard, Adam Stager, Herbert G. Tanner, Ikram Blilou, Guido Grossmann, Anjali S. Iyer-Pascuzzi, Alexis Maizel, Erin E. Sparks, Rosangela Sozzani
Annual Review of Plant Biology Year: 2020 DOI: https://doi.org/10.1146/annurev-arplant-050718-100038

Bibliography

Clark, N.M., Van den Broeck, L., Guichard, M., Stager, A., Tanner, H.G., Blilou, I., Grossmann, G., Iyer-Pascuzzi, A.S., Maizel, A., Sparks, E.E. and Sozzani, R., 2020. Novel imaging modalities shedding light on plant biology: start small and grow big. Annual review of plant biology71.

Abstract

The acquisition of quantitative information on plant development across a range of temporal and spatial scales is essential to understand the mechanisms of plant growth. Recent years have shown the emergence of imaging methodologies that enable the capture and analysis of plant growth, from the dynamics of molecules within cells to the measurement of morphometric and physiological traits in field-grown plants. In some instances, these imaging methods can be parallelized across multiple samples to increase throughput. When high throughput is combined with high temporal and spatial resolution, the resulting image-derived data sets could be combined with molecular large-scale data sets to enable unprecedented systems-level computational modeling. Such image-driven functional genomics studies may be expected to appear at an accelerating rate in the near future given the early success of the foundational efforts reviewed here. We present new imaging modalities and review how they have enabled a better understanding of plant growth from the microscopic to the macroscopic scale.

Keywords

Förster resonance energy transfer scanning fluorescent correlation spectroscopy microfluid devices light sheet microscopy multiscale imaging techniques